It can provide the theoretic basis to study the stomatal movement by regulating the expression of transcription factors
2.
The genetic regulation of stomatal movement mainly depends on an efficient control system of gene expression , and guard cell - specific promoter is becoming one of the best choices
3.
However there was little knowledge about the function of atdofl . 7 , especially to stomatal movement . we constructed plant expression vector driven by camv35s promoter and obtained tl transgenic tobaccos
4.
These include phototropism , light - driven chloroplast movements and stomatal movements . the phot i gene of arabidopsis encodes an autophosphosphorylating protein kinase that functions as a photoreceptor for phototropism in response to low - intensity bl . up to now
5.
The results represented upon observation of stomatal movement showed that the density of stoma in the epidermal of transgenic tobaccos was much lower than that of wild type and the rate of opening and closing of stoma increased because of strong expression oi atdofl . 7
6.
However , gus activity in leaves and epidermal strips increased with the prolongation of treatment time . in order to further understand the regulatory mechanism of stomatal movement , we studied one of transcription factors that might be related to stomatal movement . using rt - pcr we isolated atdofl . 7 from arabidopsis , which had a higher homology with stdofl whose expression was very high in guard cells
7.
Most interestingly , on the contrary to aba , the dcf fluorescence intensity of guard cells treated by 100 umol l - 1 salicylic acid ( sa ) was not down - regulated by pd98059 , yet pd98059 did not regulate the stomatal movement being induced by light , dark or salicylic acid
8.
Cdpks play important roles in plant calcium signal transduction . there is increasing evidence that cdpks participate in c / n metabolism , transmembrane transport of ion and water , cytoskeletal regulation , stomatal movement regulation , growth and development regulation and biotic and abiotic stress responses in plants
9.
This new method will bring significant developments in studying the principles of stomatal movement , and other quick movement in plants , c ) guard cells are incubated with ph dependent fluorescent chemical probe " bcecf am " and excited at 488nm , the fluorescent emission ratio method ( 520nm / 640nm ) is employed with laser scanning confocal microscopy , about 0 . 4 ph unit increase in guard cell vacuoles is observed during stomatal closure that is induced by aba
10.
Additionally , protein bands were found in the range of 62 kd in microsomes from oryza sativa and 45 kd from arabidopsis thaliana - , 2 ) the ipar - like protein was mainly localized in the vacuolar membrane ( tonoplast ) and plasma membrane in the leaf and root tip of arabidopsis thaliana for confirming the biological role of the ip3r - like protein , we investigated the effects of methyl viologen ( stimulating ip3 produce ) and heparin ( a competitive inhibitor of inositol 1 , 4 , 5 - triphophate in animal ) on stomatal movement by epidermal strip bioassay . when the epidermal strips of arabidopsis thaliana were treated with methyl viologen , it stimulates the stomatal closure
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